Goat anti-Mouse IgG, IgM (Whole Molecule) Antibody (HRP)

Catalog No. ABIN3045954

Quick Overview for Goat anti-Mouse IgG, IgM (Whole Molecule) Antibody (HRP) (ABIN3045954)

Target: IgG, IgM

Conjugate: HRP

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Product Details

Binding Specificity: Whole Molecule

Reactivity: Mouse

Host: Goat

Specificity: Based on IEP, this antibody reacts with:
heavy chains on mouse IgG + IgM
light chains on all mouse immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to:
non-immunoglobulin mouse serum proteins

Characteristics: Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Purification: Affinity purified using solid phase Mouse IgG + IgM

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified Mouse IgG + IgM, whole molecule

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator.
Suggested starting dilution(s): • 1:20 - 1:2,000 for most applications

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) Kathon CG

Preservative: Kathon CG

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.
Shelf Life: Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.

Target Details

Target: IgG, IgM

Alternative Name: IgG + IgM

Target Type: Antibody